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Laboratoire de physiopathologie de la barrière hémato-encéphalique The Blood-brain laboratory (EA2465) is a reseach group located in Lens at the Université d’Artois. Romeo Cecchelli and his team have a 20 year experience in blood-brain barrier research. They were the first to set up a coculture model of bovine brain capillary endothelial cells and rat glial cells that mimics the in vivo conditions. This model does not require sophisticated methods and equipment. It is already used in different academic laboratories and pharmaceutical companies and will be made available to all partners of the proposal. They are qualified to produce a state of art level in vitro culture of BBB from bovine, murine and human origins. They were the first to demonstrate the transcellular transport of blood-borne molecules using the caveolae pathway that bypass the degradative lysosomal compartment. They have expertise in “in vitro” OGD and its consequence on the integrity of the BBB and demonstrate the deleterious role of glial cells on the permeability of BBB during OGD. Taking into account their background in endothelial cell culture and coculture, they will provide, new in vitro models, (post-capillary veinule model) to the other WP working on the transport of leucocytes. They will focus their work on the role of pericytes in normal and pathological conditions on the permeability of the BBB. The group has notable experience in other European Union projects including FP6 ACUTE-TOX (WP5) and European commission contract n°CCR.IHCP. C431931.XO: 2006-2007. Contractor: Romeo Cecchelli “Evaluation of the performance of five selected in vitro blood-brain barrier for predicting absortion/uptake the brain”.
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 | Prof. Romeo Cecchelli |
Prof. Marie-Pierre Dehouck - principal investigator, experience in cell culture. Responsibility: in charge of the development of the new in vitro models – post capillary veinule and arteriolar model, triculture models of the BBB including brain capillary endothelial cells, pericytes and astrocytes.
Associate Prof. Vincent Berezowski – principal investigator, experience in cell and molecular biology. Responsibility: in charge of the transport experiment thought the BBB and its modification during OGD and reperfusion. (Identification of the glial cell signals).
Lucie Dehouck: in vitro models, preparation of syngenic mouse, KO mouse and human BBB coculture models. Immunohistochemistry and cytochemistry.
Emmanuel Sevin: Transport and OGD experiments – cell biology – confocal microscopy signal transduction and proteomic.
